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Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00028424" target="_blank" >RIV/61389030:_____/05:00028424 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation

  • Original language description

    Tobacco plants infected with the potato virus Y (PVY) were studied during the acute-infection period. The kontrol enzymes of metabolic pathway of host´s RNA degradation tending to biosynthesis of PVY-RNA, its coarse/fine regulation and content of host´sRNA were monitored. Activities of ribonucleases, phosphomonoesterases and phosphodiesterases in both the crude homogenates and the partially purified enzyme preparations from the diseased leaves were markedly increased when compared to the tissues from healthy plants. The curves of enzyme activities positively correlated with the multiplication curve of the PVY and negatively correlated with the decreased contents of host´s RNA. The enzyme activity in homogenate samples did not significantly differ fromthe corresponding purified enzyme preparations.

  • Czech name

    Regulace metabolických cest biosyntézy PVY-RNA v tabáku: degradace hostitelské RNA

  • Czech description

    Rostliny tabáku infikovaného Y-virem bramboru (PVY) byly studovány během akutní fáze infekce. Regulační enzymy degradace hostitelské RNA potřebné pro biosyntézu PVY-RNA, jejich hrubá a jemná regulace, a obsah hostitelské RNA byly monitorovány. Aktivity ribonukleáz, fosfomonoesteráz a fosfodiesteráz byly silně zvýšeny jak v hrubém homogenátu tak v částečném purifikátu daných enzymů z infikovaných listů ve srovnání s listy zdravých rostlin. Průběh enzymových aktivit negativně koreloval s obsahem hostitelské RNA. Aktivity enzymů v homogenátu se statisticky významně nelišily od aktivit v částečném purifikátu.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biologia Plantarum

  • ISSN

    0006-3134

  • e-ISSN

  • Volume of the periodical

    49

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    4

  • Pages from-to

    309-312

  • UT code for WoS article

  • EID of the result in the Scopus database