Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00030611" target="_blank" >RIV/61389030:_____/05:00030611 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition
Original language description
Glucosides of trans-zeatin occur widely in plant tissues, formed either by O-glucosylation of the hydroxylated side chain or N-glucosylation of the purine ring structure. O-Glucosylation is stereo-specific: the O-glucosyltransferase encoded by the Phaseolus lunatus ZOG1 gene has high affinity for trans-zeatin as the substrate, whereas the enzyme encoded by the maize (Zea mays) cisZOG1 gene prefers cis-zeatin. Here we show that hydroxylated derivatives of benzyladenine (topolins) are also substrates of ZOG1 and cisZOG1. The m-OH and o-OH derivatives are the preferred substrate of ZOG1 and cisZOG1, respectively. Among the hydroxylated derivatives of thidiazuron tested, the only enzyme/substrate combination resulting in conversion was cisZOG1/(o-OH) thidiazuron. The abilities of these cytokinins to serve as substrates to the glucosyltransferases were in a large part correlated with their biological activities in the P. lunatus callus bioassay, indicating that there may be similarities ...
Czech name
Topoliny a hydroxylované deriváty thidiazuronu jsou substráty cytokinin O-glukozyltransferáz, substrátová specifita těchto enzymů koreluje se specifitou cytokininových receptorů
Czech description
trans-Zeatin je v rostlinách hojně glukozylován, a to jak na hydroxylovaném postranním řetězci (O-glukozidy), tak na purinovém jádře (N-glukozidy). O-Glukozylace je stereo-specifická: O-glukozyltransferáza kódovaná genem ZOG1 z Phaseolus lunatus má vysokou afinitu vůči trans-zeatinu, zatímco enzym kódovaný genem cisZOG1 z kukuřice (Zea mays) preferuje cis-zeatin. Zjistili jsme, že substrátem pro ZOG1 a cisZOG1 jsou rovněž hydroxylované deriváty benzyladeninu (topoliny). m-OH Derivát je velmi dobrým substrátem pro ZOG1, zatímco o-OH derivát je preferován cisZOG1. Z testovaných hydroxylovaných derivátů thidiazuronu, došlo ke glukozylaci pouze u kombinace cisZOG1/(o-OH)thidiazuronu. Schopnost cytokininů interagovat s trans-zeatin O-glukozyltransferázou korelovala s jejich biologickou aktivitou ve fazolovém kalusovém biotestu, což naznačuje, že může existovat podobnost mezi cytokininovým vazebným místem enzymů a příslušných receptorů. Tento předpoklad byl potvrzen porovnáním subst...
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Physiology
ISSN
0032-0889
e-ISSN
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Volume of the periodical
137
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1057-1066
UT code for WoS article
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EID of the result in the Scopus database
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