DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F06%3A00050775" target="_blank" >RIV/61389030:_____/06:00050775 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I
Original language description
We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco (Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 °C, and (3) DNase-I. All three treatments induced a dose-dependent increase in DNA migration, expressed as percentage of tail DNA. A comparison of the fluorochrome DNA dyes ethidium bromide, DAPI and YOYO-1 demonstrated that for the alkaline version of the comet assay in plants, the commonly used fluorescent dye ethidium bromide can be used with the same efficiency as DAPI or YOYO-1.
Czech name
Barvení fluorochromy EtBr, DAPI, a YOYO v kometovém testu u tabáku po aplikaci EMS, vysoké teploty a DNase -I
Czech description
Nebyly prokázány dozdily v kometovém testu při barvení DNA pomoci 3 fluorochromů. Po aplikaci EMS, vysoké teploty a DNAsy-I bylo po alkalické elektroforéze zjištěno zvýšení poškození DNA v jádrech listů a kořenů heterozygotního tabáku Nicotiana tabacum var. xanthi. Výsledky dokazují, že kometový test je schopen detekovat jak genotoxické tak nekrotické poškození DNA.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA521%2F05%2F0500" target="_blank" >GA521/05/0500: Induced DNA damage, mutagenicity, homologous recombination and apoptosis as endpoints for monitoring effects of pollutants in tobacco and potato</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
ISSN
1383-5718
e-ISSN
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Volume of the periodical
605
Issue of the periodical within the volume
1-2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
17-21
UT code for WoS article
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EID of the result in the Scopus database
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