DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F08%3A00099655" target="_blank" >RIV/61389030:_____/08:00099655 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays

Original language description

We have calibrated the alkaline protocol of the plant comet (Single Cell Gel Electrophoresis) assay as a method for detecting the extent of induced DNA damage in potato plants (Solanum tuberosum L. cultivar Korela). After 2 and 24 h treatments of the rooted cuttings with the heavy metal cadmium (Cd2+), a dose?response increase in DNA damage was noted versus controls in root nuclei. With a 24 h recovery period, the Cd2+-induced DNA damage in roots increased significantly. No significant increase in DNA damage was demonstrated in leaf nuclei after 24 h Cd2+ treatments, but continuous Cd2+ treatments for 2 weeks resulted in an increase in leaf DNA damage. This increase may be however associated with necrotic and apoptotic DNA fragmentation, as the affected plants had inhibited growth and distorted yellowish leaves. For comparison, the monofunctional alkylating agent ethyl methanesulphonate, and ?-rays were assessed for induced DNA damage. Analysis of the accumulation of cadmium by inducti

Czech name

Poškození DNA u rostlin brambor po aplikaci kadmia, ethyl methansulfonátu a ?- paprsků

Czech description

Zakořeněné řízky brambor Solanum tuberosum kultivar Korela byly ovlivněny těžkým kovem kadmiem. Poškození DNA měřeno technikou komet, bylo prokázáno jen v kořenech rostlin brambor, zatímco v listech ke zvýšení poškození DNA nedošlo. Analýza obsahu kadmiaza použití atomového absorpčního spektrometru prokázalo, že kořený akumulují až 9x více kadmia než listy. Po aplikaci ethyl methansulfonátu a ?- paprsků došlo k poškození DNA jak v kořenech tak v listech.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA521%2F05%2F0500" target="_blank" >GA521/05/0500: Induced DNA damage, mutagenicity, homologous recombination and apoptosis as endpoints for monitoring effects of pollutants in tobacco and potato</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental and Experimental Botany

ISSN

0098-8472

e-ISSN

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Volume of the periodical

62

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

113-119

UT code for WoS article

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EID of the result in the Scopus database

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