Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F09%3A00337706" target="_blank" >RIV/61389030:_____/09:00337706 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries

Original language description

In bread wheat comprehensive genome analysis is complicated by its very large genome (17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Genetics & Breeding

ISSN

0394-9257

e-ISSN

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Volume of the periodical

61

Issue of the periodical within the volume

1-2

Country of publishing house

IT - ITALY

Number of pages

10

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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