Characterization of a new 4BS.7HL wheat?barley translocation line using GISH, FISH, and SSR markers and its effect on the ?-glucan content of wheat
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00366032" target="_blank" >RIV/61389030:_____/11:00366032 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1139/g11-044" target="_blank" >http://dx.doi.org/10.1139/g11-044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/g11-044" target="_blank" >10.1139/g11-044</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of a new 4BS.7HL wheat?barley translocation line using GISH, FISH, and SSR markers and its effect on the ?-glucan content of wheat
Original language description
A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat Asakaze komugi? barley Manas? hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitiveDNA probes (HvT01 and (AGGGAG)n) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-b-D-glucan production, was revealed in the centromeric region of 7HL.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genome
ISSN
0831-2796
e-ISSN
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Volume of the periodical
54
Issue of the periodical within the volume
10
Country of publishing house
CA - CANADA
Number of pages
10
Pages from-to
795-804
UT code for WoS article
000296987100001
EID of the result in the Scopus database
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