The Arabidopsis concentration-dependent influx/efflux transporter ABCB4 regulates cellular auxin levels in the root epidermis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00380687" target="_blank" >RIV/61389030:_____/12:00380687 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/j.1365-313X.2011.04818.x" target="_blank" >http://dx.doi.org/10.1111/j.1365-313X.2011.04818.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1365-313X.2011.04818.x" target="_blank" >10.1111/j.1365-313X.2011.04818.x</a>

Result language

angličtina

Original language name

The Arabidopsis concentration-dependent influx/efflux transporter ABCB4 regulates cellular auxin levels in the root epidermis

Original language description

Arabidopsis ATP-binding cassette B4 (ABCB4) is a root-localised auxin efflux transporter with reported auxin uptake activity in low auxin concentrations. Results reported here demonstrate that ABCB4 is a substrateactivated regulator of cellular auxin levels. The contribution of ABCB4 to shootward auxin movement at the root apex increases with auxin concentration, but in root hair elongation assays ABCB4-mediated uptake is evident at low concentrations as well. Uptake kinetics of ABCB4 heterologously expressed in Schizosaccharomyces pombe differed from the saturation kinetics of AUX1 as uptake converted to efflux at IAA concentrations. The concentration dependence of ABCB4 appears to be a direct effect on transporter activity, as ABCB4 expression and ABCB4 plasma membrane localisation at the root apex are relatively insensitive to changes in auxin concentration. Moreover, ABCB4 mediates uptake, but not efflux, of the synthetic auxin 2,4-D in cells lacking AUX1 activity. Results presente

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Journal

ISSN

0960-7412

e-ISSN

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Volume of the periodical

69

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

640-654

UT code for WoS article

000299885000007

EID of the result in the Scopus database

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