Flow cytometric chromosome sorting in plants: The next generation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00381153" target="_blank" >RIV/61389030:_____/12:00381153 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ymeth.2012.03.006" target="_blank" >http://dx.doi.org/10.1016/j.ymeth.2012.03.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ymeth.2012.03.006" target="_blank" >10.1016/j.ymeth.2012.03.006</a>

Result language
angličtina
Original language name
Flow cytometric chromosome sorting in plants: The next generation
Original language description
Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. Thiscan be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
<a href="/en/project/GAP501%2F10%2F1740" target="_blank" >GAP501/10/1740: Physical map of the wheat chromosome 4AL and positional cloning of a gene for yield</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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