Development of COS-SNP and HRM markers for high-throughput and reliable haplotype-based detection of Lr14a in durum wheat (Triticum durum Desf.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F13%3A00396707" target="_blank" >RIV/61389030:_____/13:00396707 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00122-012-2038-9" target="_blank" >http://dx.doi.org/10.1007/s00122-012-2038-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00122-012-2038-9" target="_blank" >10.1007/s00122-012-2038-9</a>

Result language

angličtina

Original language name

Development of COS-SNP and HRM markers for high-throughput and reliable haplotype-based detection of Lr14a in durum wheat (Triticum durum Desf.)

Original language description

Leaf rust (Puccinia triticina Eriks. & Henn.) is a major disease affecting durum wheat production. The Lr14a-resistant gene present in the durum wheat cv. Creso and its derivative cv. Colosseo is one of the best characterized leaf-rust resistance sourcesdeployed in durum wheat breeding. Lr14a has been mapped close to the simple sequence repeat markers gwm146, gwm344 and wmc10 in the distal portion of the chromosome arm 7BL, a gene-dense region. The objectives of this study were: (1) to enrich the Lr14aregion with single nucleotide polymorphisms (SNPs) and high-resolution melting (HRM)-based markers developed from conserved ortholog set (COS) genes and from sequenced Diversity Array Technology (DArTA (R)) markers; (2) to further investigate the gene content and colinearity of this region with the Brachypodium and rice genomes. Ten new COS-SNP and five HRM markers were mapped within an 8.0 cM interval spanning Lr14a. Two HRM markers pinpointed the locus in an interval of < 1.0 cM and e

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Theoretical and Applied Genetics

ISSN

0040-5752

e-ISSN

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Volume of the periodical

126

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

25

Pages from-to

1077-1101

UT code for WoS article

000316766000017

EID of the result in the Scopus database

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