In vivo optical detection of pH in microscopic tissue samples of Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F13%3A00424363" target="_blank" >RIV/61389030:_____/13:00424363 - isvavai.cz</a>

Alternative codes found

RIV/67985882:_____/13:00424363

Result on the web

<a href="http://dx.doi.org/10.1016/j.msec.2013.07.045" target="_blank" >http://dx.doi.org/10.1016/j.msec.2013.07.045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2013.07.045" target="_blank" >10.1016/j.msec.2013.07.045</a>

Result language

angličtina

Original language name

In vivo optical detection of pH in microscopic tissue samples of Arabidopsis thaliana

Original language description

Minimally invasive in vivo measurement of pH inmicroscopic biological samples of ?mor ?l size, e.g. plant cells, tissues and saps, may help to explain complex biological processes. Consequently, techniques to achieve such measurements are a focus of interest for botanists. This paper describes a technique for the in vivo measurement of pH in the range pH 5.0 to pH 7.8 in microscopic plant tissue samples of Arabidopsis thaliana based on a ratiometric fluorescence method using low-loss robust tapered fiber probes. For this purpose tapered fiber probes were prepared and coated with a detection layer containing ion-paired fluorescent pH-transducer 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (c-HPTS). A fluorescence ratiometric approach was employed based on excitation at 415 nm and 450 nm and on the comparison of the fluorescence response at 515 nm. The suitability of tapered fiber probes for local detection of pH between 5.0 and 7.8 was demonstrated. A pH sensitivity of 0.15 pH

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

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Project

<a href="/en/project/GAP102%2F10%2F2139" target="_blank" >GAP102/10/2139: Preparation and properties of silica-based optical glasses containing rare-earth- and chromium -doped nano-particles</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials Science & Engineering C-Materials for Biological Applications

ISSN

0928-4931

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

8

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

4809-4815

UT code for WoS article

000327686900042

EID of the result in the Scopus database

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