SNP Discovery for mapping alien introgressions in wheat

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00429596" target="_blank" >RIV/61389030:_____/14:00429596 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/1471-2164-15-273" target="_blank" >http://dx.doi.org/10.1186/1471-2164-15-273</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1471-2164-15-273" target="_blank" >10.1186/1471-2164-15-273</a>

Result language

angličtina

Original language name

SNP Discovery for mapping alien introgressions in wheat

Original language description

The short arm of chromosome 5M(g) of Ae. geniculata Roth (syn. Ae. ovata L.; 2n = 4x = 28, (UUMMg)-U-g-M-g-M-g) was flow-sorted from a wheat line in which it is maintained as a telocentric chromosome. DNA of the sorted arm was amplified and sequenced using an Illumina Hiseq 2000 with similar to 45x coverage. The sequence data was used for SNP discovery against wheat homoeologous group-5 assemblies. A total of 2,178 unique, 5M(g)S-specific SNPs were discovered. Randomly selected samples of 59 5M(g)S-specific SNPs were tested (44 by KASPar assay and 15 by Sanger sequencing) and 84% were validated. Of the selected SNPs, 97% mapped to a chromosome 5M(g) addition to wheat (the source of t5M(g)S), and 94% to 5M(g) introgressed from a different accession of Ae. geniculata substituting for chromosome 5D of wheat. The validated SNPs also identified chromosome segments of 5M(g)S origin in a set of T5D-5M(g) translocation lines; eight SNPs (25%) mapped to TA5601 [T5DL . 5DS-5M(g)S(0.75)] and thre

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

B M C Genomics

ISSN

1471-2164

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

APR 10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000334958900001

EID of the result in the Scopus database

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