Determination of Auxin Transport Parameters on the Cellular Level

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00429994" target="_blank" >RIV/61389030:_____/14:00429994 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-62703-592-7_22" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-592-7_22</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-592-7_22" target="_blank" >10.1007/978-1-62703-592-7_22</a>

Result language
angličtina
Original language name
Determination of Auxin Transport Parameters on the Cellular Level
Original language description
The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin.The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe themethod for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the ac
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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