N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00431025" target="_blank" >RIV/61389030:_____/14:00431025 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/nph.12519" target="_blank" >http://dx.doi.org/10.1111/nph.12519</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.12519" target="_blank" >10.1111/nph.12519</a>

Result language
angličtina
Original language name
N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant
Original language description
Bacteria communicate with each other in a population density-dependent process known as quorum sensing. N-acyl-homoserine lactones (HSLs) are the autoinducers of Gram-negative bacteria and the best-studied quorum sensing signals so far. HSLs induce various responses in plants, including systemic resistance and root development. Here, we used different methods, including tritium labelling, sensor strain assays and monoclonal antibodies (mAbs), to analyse the uptake and translocation of C8- and C10- homoserine lactones into barley (Hordeum vulgare cv Barke). Both HSLs were already systemically transported into the shoot at 2h after application. HSL uptake could be inhibited by orthovanadate, demonstrating that ABC transporters are involved in the uptake.Root transport occurs predominantly via the central cylinder, which was shown by transport inhibition via KCl application and autoradiography of root cross-sections. Furthermore, a newly established detection method with mAbs allowed the
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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