Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00432112" target="_blank" >RIV/61389030:_____/14:00432112 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/978-1-62703-643-6_19" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-643-6_19</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-643-6_19" target="_blank" >10.1007/978-1-62703-643-6_19</a>

Result language

angličtina

Original language name

Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes

Original language description

Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA13-19073S" target="_blank" >GA13-19073S: Multiscale analysis of signalling phospholipids and their interaction protein partners in the regulation of plant tip growth</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Plant Cell Morphogenesis: Methods and Protocols

ISBN

978-1-62703-643-6

Number of pages of the result

6

Pages from-to

231-236

Number of pages of the book

285

Publisher name

Humana Press

Place of publication

New York

UT code for WoS chapter

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