Whole-genome profiling and shotgun sequencing delivers an anchored, gene-decorated, physical map assembly of bread wheat chromosome 6A

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00432116" target="_blank" >RIV/61389030:_____/14:00432116 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/tpj.12550" target="_blank" >http://dx.doi.org/10.1111/tpj.12550</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.12550" target="_blank" >10.1111/tpj.12550</a>

Result language

angličtina

Original language name

Whole-genome profiling and shotgun sequencing delivers an anchored, gene-decorated, physical map assembly of bread wheat chromosome 6A

Original language description

Bread wheat (Triticum aestivum L.) is the most important staple food crop for 35% of the world's population. International efforts are underway to facilitate an increase in wheat production, of which the International Wheat Genome Sequencing Consortium (IWGSC) plays an important role. As part of this effort, we have developed a sequence-based physical map of wheat chromosome 6A using whole-genome profiling (WGP (TM)). The bacterial artificial chromosome (BAC) contig assembly tools FINGERPRINTED CONTIG (FPC) and LINEAR TOPOLOGICAL CONTIG (LTC) were used and their contig assemblies were compared. A detailed investigation of the contigs structure revealed that LTC created a highly robust assembly compared with those formed by FPC. The LTC assemblies contained 1217 contigs for the short arm and 1113 contigs for the long arm, with an L-50 of 1 Mb. To facilitate in silico anchoring, WGP (TM) tags underlying BAC contigs were extended by wheat and wheat progenitor genome sequence information.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Journal

ISSN

0960-7412

e-ISSN

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Volume of the periodical

79

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

334-347

UT code for WoS article

000339439200013

EID of the result in the Scopus database

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