Common Wheat Chromosome 5B Composition Analysis Using Low-Coverage 454 Sequencing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00433557" target="_blank" >RIV/61389030:_____/14:00433557 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3835/plantgenome2013.10.0031" target="_blank" >http://dx.doi.org/10.3835/plantgenome2013.10.0031</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3835/plantgenome2013.10.0031" target="_blank" >10.3835/plantgenome2013.10.0031</a>

Result language

angličtina

Original language name

Common Wheat Chromosome 5B Composition Analysis Using Low-Coverage 454 Sequencing

Original language description

The sequencing of individual chromosomes of common wheat is in progress. The molecular size of wheat chromosome 5B is nearly 870 Mb (5BL = 580 Mb and 5BS = 290 Mb). We produced the first low coverage 454-sequencing of the long and short arms of wheat chromosome 5B (110,793 and 39,695 reads, which compose 8 and 6% of total 5BL and 5BS length, respectively) and calculated the ratios of the different families of repetitive sequences, including transposable elements (TEs), satellite repeats (Afa, pSc119.2,5S rDNA and 45S rDNA), and microsatellites, as well as direct and inverted repeat motifs. The TEs accounted for 70% of the total analyzed nucleotide sequences. The content of the Cereba retrotransposon family differed between the two arms of chromosome 5B. Comparing the reads of chromosome 5B with the data from chromosome 5A, we found the retrotransposons Fatima and Sakura and DNA transposon Jorge were prevalent in 5B. The hypothetical coding sequences accounted for 2.0% of the short arm

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Genome

ISSN

1940-3372

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

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UT code for WoS article

000338834700002

EID of the result in the Scopus database

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