Next-generation sequencing of flow-sorted wheat chromosome 5D reveals lineage-specific translocations and widespread gene duplications

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00441332" target="_blank" >RIV/61389030:_____/14:00441332 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/1471-2164-15-1080" target="_blank" >http://dx.doi.org/10.1186/1471-2164-15-1080</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/1471-2164-15-1080" target="_blank" >10.1186/1471-2164-15-1080</a>

Result language
angličtina
Original language name
Next-generation sequencing of flow-sorted wheat chromosome 5D reveals lineage-specific translocations and widespread gene duplications
Original language description
Background: The similar to 17 Gb hexaploid bread wheat genome is a high priority and a major technical challenge for genomic studies. In particular, the D sub-genome is relatively lacking in genetic diversity, making it both difficult to map genetically,and a target for introgression of agriculturally useful traits. Elucidating its sequence and structure will therefore facilitate wheat breeding and crop improvement. Results: We generated shotgun sequences from each arm of flow-sorted Triticum aestivumchromosome 5D using 454 FLX Titanium technology, giving 1.34x and 1.61x coverage of the short (5DS) and long (5DL) arms of the chromosome respectively. By a combination of sequence similarity and assembly-based methods, similar to 74% of the sequence reads were classified as repetitive elements, and coding sequence models of 1314 (5DS) and 2975 (5DL) genes were generated. The order of conserved genes in syntenic regions of previously sequenced grass genomes were integrated with physical
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GBP501%2F12%2FG090" target="_blank" >GBP501/12/G090: Evolution and Function of Complex Plant Genomes</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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