Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00446311" target="_blank" >RIV/61389030:_____/15:00446311 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3389/fpls.2015.00066" target="_blank" >http://dx.doi.org/10.3389/fpls.2015.00066</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2015.00066" target="_blank" >10.3389/fpls.2015.00066</a>

Result language

angličtina

Original language name

Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana

Original language description

Aluminum ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. The first indication of the Al toxicity in plants is the cessation of root growth, but the mechanism of root growth inhibition is largely unknown. Here we examined the impact of Al on the expression, activity, and function of the non-specific phospholipase C4 (NPC4), a plasma membrane-bound isoform of NPC, a member of the plant phospholipase family, in Arabidopsis thaliana. We observed a lower expressionof NPC4 using beta-glucuronidase assay and a decreased formation of labeled diacylglycerol, product of NPC activity, using fluorescently labeled phosphatidylcholine as a phospholipase substrate in Arabidopsis WT seedlings treated with AlCl3 for 2 h. Theeffect on in situ NPC activity persisted for longer Al treatment periods (8, 14 h). Interestingly, in seedlings overexpressing NPC4, the Al-mediated NPC-inhibiting effect was alleviated at 14 h. However, in vitro activity and localizatio

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GPP501%2F12%2FP950" target="_blank" >GPP501/12/P950: The role of diacylglycerol in aluminium toxicity in plants</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

FEB 16 2015

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

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UT code for WoS article

000349641900001

EID of the result in the Scopus database

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