Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00451395" target="_blank" >RIV/61389030:_____/15:00451395 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/15:00447664

Result on the web

<a href="http://dx.doi.org/10.1186/s12864-015-1698-7" target="_blank" >http://dx.doi.org/10.1186/s12864-015-1698-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-015-1698-7" target="_blank" >10.1186/s12864-015-1698-7</a>

Result language

angličtina

Original language name

Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia

Original language description

Silene latifolia represents one of the best-studied plant sex chromosome systems. A new approach using RNA-seq data has recently identified hundreds of new sex-linked genes in this species. However, this approach is expected to miss genes that are eithernot expressed or are expressed at low levels in the tissue(s) used for RNA-seq. Therefore other independent approaches are needed to discover such sex-linked genes. Here we used 10 well-characterized S. latifolia sex-linked genes and their homologs in Silene vulgaris, a species without sex chromosomes, to screen BAC libraries of both species. We isolated and sequenced 4 Mb of BAC clones of S. latifolia X and Y and S. vulgaris genomic regions, which yielded 59 new sex-linked genes (with S. vulgaris homologs for some of them). We assembled sequences that we believe represent the tip of the Xq arm. These sequences are clearly not pseudoautosomal, so we infer that the S. latifolia X has a single pseudoautosomal region (PAR) on the Xp arm.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GAP501%2F12%2F2220" target="_blank" >GAP501/12/2220: Sex chromosome evolution - chromosome-specific genomics in genus Silene</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

B M C Genomics

ISSN

1471-2164

e-ISSN

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Volume of the periodical

16

Issue of the periodical within the volume

JUL 25

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

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UT code for WoS article

000358638500001

EID of the result in the Scopus database

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