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Metabolic changes and associated cytokinin signals in response to nitrate assimilation in roots and shoots of Lolium perenne

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00460033" target="_blank" >RIV/61389030:_____/16:00460033 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15310/16:33161903

  • Result on the web

    <a href="http://dx.doi.org/10.1111/ppl.12412" target="_blank" >http://dx.doi.org/10.1111/ppl.12412</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/ppl.12412" target="_blank" >10.1111/ppl.12412</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Metabolic changes and associated cytokinin signals in response to nitrate assimilation in roots and shoots of Lolium perenne

  • Original language description

    The efficiency of inorganic nitrogen (N) assimilation is a critical component of fertilizer use by plants and of forage production in Lolium perenne, an important pasture species worldwide. We present a spatiotemporal description of nitrate use efficiency in terms of metabolic responses and carbohydrate remobilization, together with components of cytokinin signal transduction following nitrate addition to N-impoverished plants. Perennial ryegrass (L. perenne cv. Grasslands Nui) plants were grown for 10weeks in unfertilized soil and then treated with nitrate (5mM) hydroponically. Metabolomic analysis by gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry revealed a dynamic interaction between N and carbon metabolism over a week-long time course represented by the relative abundance of amino acids, tricarboxylic acid intermediates and stored water-soluble carbohydrates (WSCs). The initial response to N addition was characterized by a rapid remobilization of carbon stores from the low-molecular weight WSC, along with an increase in N content and assimilation into free amino acids. Subsequently, the shoot became the main source of carbon through remobilization of a large pool of high-molecular weight WSC. Associated quantification of cytokinin levels and expression profiling of putative cytokinin response regulator genes by quantitative reverse transcription polymerase chain reaction support a role for cytokinin in the mediation of the response to N addition in perennial ryegrass. The presence of high levels of cis-zeatin-type cytokinins is discussed in the context of hormonal homeostasis under the stress of steady-state N deficiency.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physiologia Plantarum

  • ISSN

    0031-9317

  • e-ISSN

  • Volume of the periodical

    156

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    497-511

  • UT code for WoS article

    000372974400009

  • EID of the result in the Scopus database