The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00461519" target="_blank" >RIV/61389030:_____/16:00461519 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/s13007-016-0124-8" target="_blank" >http://dx.doi.org/10.1186/s13007-016-0124-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13007-016-0124-8" target="_blank" >10.1186/s13007-016-0124-8</a>

Result language
angličtina
Original language name
The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat
Original language description
Background: Identification of transgene insertion sites in plant genomes has practical implications for crop breeding and is a stepping stone to analyze transgene function. However, single copy sequences are not always easy to localize in large plant genomes by standard approaches. nResults: We employed flow cytometric chromosome sorting to determine chromosomal location of barley sucrose transporter construct in three transgenic lines of common wheat. Flow-sorted chromosomes were used as template for PCR and fluorescence in situ hybridization to identify chromosomes with transgenes. The chromosomes carrying the transgenes were then confirmed by PCR using DNA amplified from single flow-sorted chromosomes as template. nConclusions: Insertion sites of the transgene were unambiguously localized to chromosomes 4A, 7A and 5D in three wheat transgenic lines. The procedure presented in this study is applicable for localization of any single-copy sequence not only in wheat, but in any plant species where suspension of intact mitotic chromosomes suitable for flow cytometric sorting can be prepared.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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