Leptosphaeria maculans effector AvrLm4-7 affects salicylic acid (SA) and ethylene (ET) signalling and hydrogen peroxide (H2O2) accumulation in Brassica napus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00462768" target="_blank" >RIV/61389030:_____/16:00462768 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10326234
Result on the web
<a href="http://dx.doi.org/10.1111/mpp.12332" target="_blank" >http://dx.doi.org/10.1111/mpp.12332</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/mpp.12332" target="_blank" >10.1111/mpp.12332</a>
Alternative languages
Result language
angličtina
Original language name
Leptosphaeria maculans effector AvrLm4-7 affects salicylic acid (SA) and ethylene (ET) signalling and hydrogen peroxide (H2O2) accumulation in Brassica napus
Original language description
To achieve host colonization, successful pathogens need to overcome plant basal defences. For this, (hemi)biotrophic pathogens secrete effectors that interfere with a range of physiological processes of the host plant. AvrLm4-7 is one of the cloned effectors from the hemibiotrophic fungus Leptosphaeria maculans brassicaceae' infecting mainly oilseed rape (Brassica napus). Although its mode of action is still unknown, AvrLm4-7 is strongly involved in L. maculans virulence. Here, we investigated the effect of AvrLm4-7 on plant defence responses in a susceptible cultivar of B. napus. Using two isogenic L. maculans isolates differing in the presence of a functional AvrLm4-7 allele [absence (a4a7') and presence (A4A7') of the allele], the plant hormone concentrations, defence-related gene transcription and reactive oxygen species (ROS) accumulation were analysed in infected B. napus cotyledons. Various components of the plant immune system were affected. Infection with the A4A7' isolate caused suppression of salicylic acid- and ethylene-dependent signalling, the pathways regulating an effective defence against L. maculans infection. Furthermore, ROS accumulation was decreased in cotyledons infected with the A4A7' isolate. Treatment with an antioxidant agent, ascorbic acid, increased the aggressiveness of the a4a7' L. maculans isolate, but not that of the A4A7' isolate. Together, our results suggest that the increased aggressiveness of the A4A7' L. maculans isolate could be caused by defects in ROS-dependent defence and/or linked to suppressed SA and ET signalling. This is the first study to provide insights into the manipulation of B. napus defence responses by an effector of L. maculans.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-26798S" target="_blank" >GA13-26798S: Fungal effectors manipulating plant defence system</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Plant Pathology
ISSN
1464-6722
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
818-831
UT code for WoS article
000379948300004
EID of the result in the Scopus database
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