Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00465513" target="_blank" >RIV/61389030:_____/16:00465513 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/s13059-016-1082-1" target="_blank" >http://dx.doi.org/10.1186/s13059-016-1082-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13059-016-1082-1" target="_blank" >10.1186/s13059-016-1082-1</a>

Result language
angličtina
Original language name
Rapid gene isolation in barley and wheat by mutant chromosome sequencing
Original language description
Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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