Auxin-cytokinin interaction and variations in their metabolic products in the regulation of organogenesis in two Eucomis species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00465915" target="_blank" >RIV/61389030:_____/16:00465915 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/16:33161081
Result on the web
<a href="http://dx.doi.org/10.1016/j.nbt.2016.09.001" target="_blank" >http://dx.doi.org/10.1016/j.nbt.2016.09.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nbt.2016.09.001" target="_blank" >10.1016/j.nbt.2016.09.001</a>
Alternative languages
Result language
angličtina
Original language name
Auxin-cytokinin interaction and variations in their metabolic products in the regulation of organogenesis in two Eucomis species
Original language description
In the current study, we evaluated the effect of alpha-naphthaleneacetic acid (NAA) individually or in combination with different cytokinins (CKs) including benzyladenine (BA), meta-topolin (mT) and isopentenyladenine (iP) on organogenesis, auxin and CK content in Eucomis autumnalis subspecies autumnalis (EA) and Eucomis zambesiaca (EZ). These species were used as model plants due to their ornamental and medicinal properties. Three leaf explants were inoculated in screw-cap jars containing 30 mL Murashige and Skoog (MS) media supplemented with 5 mM NAA alone or in combination with 5 mu M CK (BA, mT or iP). After 10 weeks (EA) or 15 weeks (EZ), parameters including shoot and root growth as well as plant fresh weight were recorded. For analysis of auxin and CK content, whole plantlets were harvested, pooled and freeze-dried for the different treatments. In both species, shoot and root proliferation as well as plant biomass were generally higher when NAA was combined with the individual CK than in NAA or CK treatment. The highest concentration of indole-3-acetic acid (IAA, 619 pmol g(-1) DW) and 2-oxindole-3-acetic acid (OxIAA, 2381 pmol g(-1) DW) were observed in EA-treated with NAA alone while mT treatment (without NAA) had the most abundant indole-3-acetyl-L-aspartic acid (IAAsp, 904 and 582 pmol g(-1) DW for EA and EZ, respectively) in both species. A significant concentration of total endogenous CK accumulated in both Eucomis regenerants from mT and mT + NAA when compared to the other treatments. The majority of the detected CKs were of the aromatic CK-type, mainly free bases. The potential physiological roles of these quantified phytohormones in relation to the observed morphological responses are discussed. (C) 2016 Elsevier B.V. All rights reserved.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Biotechnology
ISSN
1871-6784
e-ISSN
—
Volume of the periodical
33
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
883-890
UT code for WoS article
000386782600016
EID of the result in the Scopus database
—