Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00467448" target="_blank" >RIV/61389030:_____/16:00467448 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.plantsci.2016.09.008" target="_blank" >http://dx.doi.org/10.1016/j.plantsci.2016.09.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2016.09.008" target="_blank" >10.1016/j.plantsci.2016.09.008</a>

Result language

angličtina

Original language name

Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants

Original language description

In plants, indole-3-acetic acid (IAA) amido hydrolases (AHs) participate in auxin homeostasis by releasing free IAA from IAA-amino acid conjugates. We investigated the role of IAR3, a member of the IAA amido hydrolase family, in the response of Solanaceous plants challenged by biotrophic and hemibiotrophic pathogens. By means of genome inspection and phylogenic analysis we firstly identified IAA-AH sequences and putative IAR3 orthologs in Nicotiana benthamiana, tomato and potato. We evaluated the involvement of IAR3 genes in defense responses by using virus-induced gene silencing. We observed that N. benthamiana and tomato plants with knocked-down expression of IAR3 genes contained lower levels of free IAA and presented altered responses to pathogen attack, including enhanced basal defenses and higher tolerance to infection in susceptible plants. We showed that IAR3 genes are consistently up-regulated in N. benthamiana and tomato upon inoculation with Phytophthora infestans and Cladosporium fulvum respectively. However, IAR3 expression decreased significantly when hypersensitive response was triggered in transgenic tomato plants coexpressing the Cf-4 resistance gene and the avirulence factor Avr4. Altogether, our results indicate that changes in IAR3 expression lead to alteration in auxin homeostasis that ultimately affects plant defense responses.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

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Volume of the periodical

253

Issue of the periodical within the volume

DEC

Country of publishing house

IE - IRELAND

Number of pages

9

Pages from-to

31-39

UT code for WoS article

000388049000004

EID of the result in the Scopus database

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