Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00475872" target="_blank" >RIV/61389030:_____/17:00475872 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/17:73584680

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0170715" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0170715</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0170715" target="_blank" >10.1371/journal.pone.0170715</a>

Result language

angličtina

Original language name

Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant

Original language description

The 78-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 78-1, transcriptomic profiles of the 78-1 malesterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 upregulated and 636 downregulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 78-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 78-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 78-1 anthers. The present study provides insights into the transcriptome of the 78-1 mutant. We identified several genes with altered expression level in 78-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

19

Pages from-to

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UT code for WoS article

000393712500013

EID of the result in the Scopus database

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