Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476491" target="_blank" >RIV/61389030:_____/17:00476491 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/sdata.2017.44" target="_blank" >http://dx.doi.org/10.1038/sdata.2017.44</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/sdata.2017.44" target="_blank" >10.1038/sdata.2017.44</a>

Result language

angličtina

Original language name

Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.

Original language description

Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. a Morex was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SCIENTIFIC DATA

ISSN

2052-4463

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

APR 27

Country of publishing house

GB - UNITED KINGDOM

Number of pages

24

Pages from-to

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UT code for WoS article

000400150800001

EID of the result in the Scopus database

2-s2.0-85018297524