Infection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476550" target="_blank" >RIV/61389030:_____/17:00476550 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/17:73579603
Result on the web
<a href="http://dx.doi.org/10.1093/aob/mcw202" target="_blank" >http://dx.doi.org/10.1093/aob/mcw202</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/aob/mcw202" target="_blank" >10.1093/aob/mcw202</a>
Alternative languages
Result language
angličtina
Original language name
Infection by rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen
Original language description
Background and Aims: Pisum sativum L. (pea) seed is a source of carbohydrate and protein for the developing plant. By studying pea seeds inoculated by the cytokinin-producing bacterium, Rhodococcus fascians, we sought to determine the impact of both an epiphytic (avirulent) strain and a pathogenic strain on source-sink activity within the cotyledons during and following germination.nMethods: Bacterial spread was monitored microscopically, and real-time reverse transcription-quantitative PCR was used to determine the expression of cytokinin biosynthesis, degradation and response regulator gene family members, along with expression of family members of SWEET, SUT, CWINV and AAP genes gene families identified initially in pea by transcriptomic analysis. The endogenous cytokinin content was also determined.nKey Results: The cotyledons infected by the virulent strain remained intact and turned green, while multiple shoots were formed and root growth was reduced. The epiphytic strain had no such marked impact. Isopentenyl adenine was elevated in the cotyledon s infected by the virulent strain. Strong expression of RfIPT, RfLOG and RfCKX was detected in the cotyledons infected by the virulent strain throughout the experiment, with elevated expression also observed for PsSWEET, PsSUT and PsINV gene family members. The epiphytic strain had some impact on the expression of these genes, especially at the later stages of reserve mobilization from the cotyledons.nConclusions: The pathogenic strain retained the cotyledons as a sink tissue for the pathogen rather than the cotyledon converting completely to a source tissue for the germinating plant. We suggest that the interaction of cytoki-nins, CWINVs and SWEETs may lead to the loss of apical dominance and the appearance of multiple shoots.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Annals of Botany
ISSN
0305-7364
e-ISSN
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Volume of the periodical
119
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
841-852
UT code for WoS article
000400982600013
EID of the result in the Scopus database
2-s2.0-85018991925