Subcellular localization of Arabidopsis pathogenesis-related 1 (PR1) protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476648" target="_blank" >RIV/61389030:_____/17:00476648 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/17:10360279
Result on the web
<a href="http://dx.doi.org/10.3390/ijms18040825" target="_blank" >http://dx.doi.org/10.3390/ijms18040825</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms18040825" target="_blank" >10.3390/ijms18040825</a>
Alternative languages
Result language
angličtina
Original language name
Subcellular localization of Arabidopsis pathogenesis-related 1 (PR1) protein
Original language description
The Arabidopsis thaliana pathogenesis-related 1 (PR1) is an important defense protein, so far it has only been detected in extracellular space and its subcellular sorting and transport remain unexplained. Using a green fluorescent protein (GFP) tagged full length, as well as a C-terminus truncated version of PR1, we observed that when expressed ectopically in Nicotiana benthamiana leaves, PR1 co-localizes only partially with Golgi markers, and much more prominently with the late endosome (LE)/multivesicular body (MVB) FYVE marker. The C-truncated version PR1∆C predominantly localized to the endoplasmic reticulum (ER). The same localizations were found for stable Arabidopsis transformants with expression of PR1 and PR1∆C driven by the native promoter. We conclude that the A. thaliana PR1 (AtPR1) undergoes an unconventional secretion pathway, starting from the C-terminus-dependent sorting from the ER, and utilizing further transportation via phosphatidyl-inositol-3-phosphate (PI(3)P) positive LE/MVB-like vesicles. The homology model of the PR1 structure shows that the cluster of positively charged amino acid residues (arginines 60, 67, 137, and lysine 135) could indeed interact with negatively charged phospholipids of cellular membranes. It remains to be resolved whether Golgi and LE/MVB localization reflects an alternative sorting or trafficking succession, and what the role of lipid interactions in it will be.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
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UT code for WoS article
000402639400149
EID of the result in the Scopus database
2-s2.0-85018498280