Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00482335" target="_blank" >RIV/61389030:_____/17:00482335 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/nbt.3877" target="_blank" >http://dx.doi.org/10.1038/nbt.3877</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nbt.3877" target="_blank" >10.1038/nbt.3877</a>

Result language

angličtina

Original language name

Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

Original language description

Cereal crops such as wheat and maize have large repeat-rich genomes that make cloning of individual genes challenging. Moreover, gene order and gene sequences often differ substantially between cultivars of the same crop species1-4. A major bottleneck for gene cloning in cereals is the generation of high-quality sequence information from a cultivar of interest. In order to accelerate gene cloning from any cropping line, we report ` targeted chromosome-based cloning via long-range assembly' (TACCA). TACCA combines lossless genome-complexity reduction via chromosome flow sorting with Chicago long-range linkage5 to assemble complex genomes. We applied TACCA to produce a high-quality (N50 of 9.76 Mb) de novo chromosome assembly of the wheat line CH Campala Lr22a in only 4 months. Using this assembly we cloned the broad-spectrum Lr22a leaf-rust resistance gene, using molecular marker information and ethyl methanesulfonate (EMS) mutants, and found that Lr22a encodes an intracellular immune receptor homologous to the Arabidopsis thaliana RPM1 protein.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Biotechnology

ISSN

1087-0156

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

793-796

UT code for WoS article

000407125800028

EID of the result in the Scopus database

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