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24-Epibrassinolide as a Modifier of Antioxidant Activities and Membrane Properties of Wheat Cells in Zearalenone Stress Conditions

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00497160" target="_blank" >RIV/61389030:_____/18:00497160 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15310/18:73591428

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s00344-018-9792-0" target="_blank" >http://dx.doi.org/10.1007/s00344-018-9792-0</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00344-018-9792-0" target="_blank" >10.1007/s00344-018-9792-0</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    24-Epibrassinolide as a Modifier of Antioxidant Activities and Membrane Properties of Wheat Cells in Zearalenone Stress Conditions

  • Original language description

    The mechanism of action of brassinosteroids (BRs) in plant cells under stress has not been fully explained, despite ample evidence of their protective effects. The aim of this study was to investigate the significance of physicochemical properties of cell membranes during an interaction with BRs under stress conditions induced by a mycotoxin zearalenone (ZEA). Experiments were performed in in vitro cultures of wheat cells obtained from immature embryos of tolerant and sensitive genotypes. ZEA added to media (30 µM) accumulated in greater amounts in the cells of sensitive wheat, contrary to BRs, which accumulated in greater amounts by the tolerant genotype when added to media at 0.1 µM. Incorporation of 24-epibrassinolide (EBR) stimulated synthesis of casta- and homocastasterone, that is, endogenous BRs present in wheat cells, and enhanced the content of homocastasterone. When the cultures were supplemented with the mixture of ZEA and EBR, castasterone synthesis was stimulated to a higher degree in cells of the sensitive plant. EBR and ZEA added separately activated antioxidant enzyme systems in both genotypes but with preference for the sensitive one. In the cells treated with ZEA + EBR, the activation was close to that observed for EBR alone. The study discussed also the role of membrane permeability, electrokinetic potential changes, and structural properties of native (plasmalemma) and model (DOPC) monolayers in the mechanism of EBR-induced protection, including the possibility of replacing ZEA absorbed in the membrane lipid layers by BR molecules, independently of the activation of the antioxidant system.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10611 - Plant sciences, botany

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Plant Growth Regulation

  • ISSN

    0721-7595

  • e-ISSN

  • Volume of the periodical

    37

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

    1085-1098

  • UT code for WoS article

    000451974000006

  • EID of the result in the Scopus database

    2-s2.0-85044918151