Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00501610" target="_blank" >RIV/61389030:_____/19:00501610 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/19:73594175

Result on the web

<a href="http://dx.doi.org/10.1016/j.nbt.2018.03.003" target="_blank" >http://dx.doi.org/10.1016/j.nbt.2018.03.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.nbt.2018.03.003" target="_blank" >10.1016/j.nbt.2018.03.003</a>

Result language

angličtina

Original language name

Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

Original language description

Bread wheat (Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits and provide a basis for genomics-assisted breeding. Physical maps and BAC clones associated with them have been valuable resources from which to generate a reference genome of bread wheat and to assist map-based gene cloning. As a part of a joint effort coordinated by the International Wheat Genome Sequencing Consortium, we have constructed a BAC-based physical map of bread wheat chromosome arm 7DS consisting of 895 contigs and covering 94% of its estimated length. By anchoring BAC contigs to one radiation hybrid map and three high resolution genetic maps, we assigned 73% of the assembly to a distinct genomic position. This map integration, interconnecting a total of 1713 markers with ordered and sequenced BAC clones from a minimal tiling path, provides a tool to speed up gene cloning in wheat. The process of physical map assembly included the integration of the 7DS physical map with a whole-genome physical map of Aegilops tauschii and a 7DS Bionano genome map, which together enabled efficient scaffolding of physical-map contigs, even in the non-recombining region of the genetic centromere. Moreover, this approach facilitated a comparison of bread wheat and its ancestor at BAC-contig level and revealed a reconstructed region in the 7DS pericentromere.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

New Biotechnology

ISSN

1871-6784

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

JAN 25

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

12-19

UT code for WoS article

000450366200003

EID of the result in the Scopus database

2-s2.0-85044298699