Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00502522" target="_blank" >RIV/61389030:_____/19:00502522 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/19:73598557

Result on the web

<a href="http://dx.doi.org/10.1186/s12870-019-1657-6" target="_blank" >http://dx.doi.org/10.1186/s12870-019-1657-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12870-019-1657-6" target="_blank" >10.1186/s12870-019-1657-6</a>

Result language

angličtina

Original language name

Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity

Original language description

Background: Cytokinin is a negative regulator of root growth, and a reduction of the cytokinin content or signalling causes the formation a larger root system in model plants, improves their growth under drought and nutrient limitation and causes increased accumulation of elements in the shoot. Roots are an important but understudied target of plant breeding. Here we have therefore explored whether root enhancement by lowering the cytokinin content can also be achieved in oilseed rape (Brassica napus L.) plants. Results: Transgenic plants overexpressing the cKX2 gene of Arabidopsis thaliana encoding a cytokinin-degrading cytokinin oxidase/dehydrogenase showed higher cKX activity and a strongly reduced cytokinin content. Cytokinin deficiency led to the formation of a larger root system under different growth conditions, which was mainly due to an increased number of lateral and adventitious roots. In contrast, shoot growth was comparable to wild type, which caused an enhanced root-to-shoot ratio. Transgenic plants accumulated in their leaves higher concentrations of macro- And microelements including P, ca, Mg, S, Zn, cu, Mo and Mn. They formed more chlorophyll under Mg- And S-deficiency and accumulated a larger amount of cd and Zn from contaminated medium and soil. Conclusions: These findings demonstrate the usefulness of ectopic cKX gene expression to achieve root enhancement in oilseed rape and underpin the functional relevance of a larger root system. Furthermore, the lack of major developmental consequences on shoot growth in cytokinin-deficient oilseed rape indicates species-specific differences of cKX gene and/or cytokinin action.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Plant Biology

ISSN

1471-2229

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

FEB 20

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

000459388100002

EID of the result in the Scopus database

2-s2.0-85061964749