Strong antioxidant capacity of horseradish hairy root cultures under arsenic stress indicates the possible use of Armoracia rusticana plants for phytoremediation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00504122" target="_blank" >RIV/61389030:_____/19:00504122 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/19:10393721

Result on the web

<a href="http://doi.org/10.1016/j.ecoenv.2019.02.028" target="_blank" >http://doi.org/10.1016/j.ecoenv.2019.02.028</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecoenv.2019.02.028" target="_blank" >10.1016/j.ecoenv.2019.02.028</a>

Result language

angličtina

Original language name

Strong antioxidant capacity of horseradish hairy root cultures under arsenic stress indicates the possible use of Armoracia rusticana plants for phytoremediation

Original language description

The potential contamination of the food chain is the most important aspect of arsenic (As) pollution, since it is highly toxic to all organisms. Thus, the search for As hyperaccumulators suitable to remove As from contaminated soils appears to be a vital task. Horseradish (Armoracia rusticana), a crop plant with a high potential to accumulate heavy metals, can also serve to study the physiological processes that accompany arsenic stress. The significant adverse effect caused by arsenic exposure is an oxidative stress. Plants have developed a highly organized system to quench free radicals, which includes the action of both enzymatic and non-enzymatic quenching. Saccharides are proposed to possess outstanding antioxidant activity in vitro, and thus, they are likely to effectively quench free radicals also in plant tissues. In this study, root cultures (hairy root type) of horseradish were grown in vitro on media with different concentrations of arsenic (5–60 µg l −1 ). Arsenic slowed down the growth, nevertheless up to three-fold biomass increase was achieved at the highest dose. Moreover, root tissues were able to remove as much as 75% of arsenic from the cultivation medium within 7 days. We also evaluated diverse oxidative-stress-related features: contents of reactive oxygen species, the activities of key antioxidant enzymes, and the contents of important antioxidant molecules, such as glutathione, proline, phenolic compounds and non-structural carbohydrates. At all arsenic treatments, we observed a significant proline content increase and enhanced antioxidant enzymes (peroxidase, catalase and glutathione-S-transpherase) activities peaking, however, at different doses. Soluble carbohydrates contents also significantly increased after 7-day treatment a then decreased nearly to the original levels. This study points to efficient antioxidant system of horseradish hairy roots enabling good growth and substantial As accumulation even under high As exposure. Providing that horseradish shares these important features with this model system, we could propose that horseradish is a promising candidate to exploit in arsenic phytoremediation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Project

<a href="/en/project/LO1417" target="_blank" >LO1417: Centre of Experimental Plant Biology of CU</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Ecotoxicology and Enviromental Safety

ISSN

0147-6513

e-ISSN

—

Volume of the periodical

174

Issue of the periodical within the volume

JUN 15

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

295-304

UT code for WoS article

000463465600033

EID of the result in the Scopus database

2-s2.0-85062350993