Exogenous N-Acetylcysteine alleviates heavy metal stress by promoting phenolic acids to support antioxidant defence systems in wheat roots

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00507565" target="_blank" >RIV/61389030:_____/19:00507565 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/19:73598437

Result on the web

<a href="http://dx.doi.org/10.1016/j.ecoenv.2019.05.052" target="_blank" >http://dx.doi.org/10.1016/j.ecoenv.2019.05.052</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecoenv.2019.05.052" target="_blank" >10.1016/j.ecoenv.2019.05.052</a>

Result language

angličtina

Original language name

Exogenous N-Acetylcysteine alleviates heavy metal stress by promoting phenolic acids to support antioxidant defence systems in wheat roots

Original language description

N-acetylcysteine (N-Acetyl L-cysteine, NAC) is a thiol compound derived from the addition of the acetyl group to cysteine amino acid. NAC has been used as an antioxidant, free radical scavenger, and chelating agent for reducing the deleterious effects on plants of biotic and abiotic environmental stresses. It can also relieve heavy metal (HM) toxicity, although its alleviating mechanism remains unknown. In this study, we compared HM-stressed (Cu, Hg, Cd and Pb, 100 μM each) wheat seedlings without NAC treatment and in combination with NAC (1 mM). In comparison to HMs alone, NAC treatment in combination with HMs (Cu, Cd, Hg and Pb, respectively) stimulated root growth (1.1-, 1.5-, 10.5- and 1.9-fold), and significantly increased fresh (1.3-, 1.5-, 4.3- and 1.4-fold) and dry (1.2-, 1.5-, 2.5- and 1.2-fold) mass. Combination treatment also led to significant reductions in HM concentrations (1.3-, 1.4-, 4- and 1.1-fold, respectively). GSH (1.1 – 1.8-fold), TBARS (1.4 – 2.7-fold) and H2O2 (1.6 – 1.8-fold) contents in treatment with HMs alone were significantly mitigated by the NAC combination. Some of the antioxidant enzyme activities increased or reduced by some HM treatments alone were stimulated by a combination of NAC with HMs, or remained unchanged or changed only insignificantly, supported by the phenolic pool of the plant. Ferulic, p-comaric and syringic acids were the major phenolic acids (PAs) in the roots in free, ester, glycoside and ester-bound forms, and their concentrations were increased by HM treatments alone, in comparison to the control seedlings, while PAs concentrations were relatively reduced by NAC in combination with HMs. These results indicate that NAC can alleviate HM toxicity and improve the growth of HM-stressed wheat seedlings by coordinated induction of the phenolic pool and the antioxidant defence system.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Ecotoxicology and Enviromental Safety

ISSN

0147-6513

e-ISSN

—

Volume of the periodical

181

Issue of the periodical within the volume

OCT 15

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

49-59

UT code for WoS article

000475411500007

EID of the result in the Scopus database

2-s2.0-85066410980