Quantification of karrikins in smoke water using ultra-high performance liquid chromatography-tandem mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00507849" target="_blank" >RIV/61389030:_____/19:00507849 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/19:73598508
Result on the web
<a href="http://dx.doi.org/10.1186/s13007-019-0467-z" target="_blank" >http://dx.doi.org/10.1186/s13007-019-0467-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13007-019-0467-z" target="_blank" >10.1186/s13007-019-0467-z</a>
Alternative languages
Result language
angličtina
Original language name
Quantification of karrikins in smoke water using ultra-high performance liquid chromatography-tandem mass spectrometry
Original language description
Karrikins (KARs) are plant growth regulators that promote seed germination and the subsequent growth and development of seedlings of many plant species. In nature they are generated and released by combustion of plant material and promote the restoration of burned ecosystems. Smoke water can be artificially prepared as a saturated extract of all substances in smoke produced by burning plants, and it has various horticultural and agricultural applications. Results: We have developed, validated and applied the first fast, specific and sensitive method, based on ultra-high performance liquid chromatography-tandem mass spectrometry, for quantifying KARs in smoke water. To assist these efforts and further analyses, standards of the main KARs (which are not commercially available) were synthesized. Due to the complex matrix of smoke waters, two quantification approaches (standard dilution with a structural KAR analogue and standard addition) were compared. The standard addition method allowed absolute quantification of KARs in six of eight smoke water samples of diverse origins and ages. Conclusions: Our findings reveal differences in both total and relative levels of KARs in smoke water, and indicate that differences in its KAR composition may be linked to variations in its bioactivity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Methods
ISSN
1746-4811
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
JUL 25
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
81
UT code for WoS article
000477594100002
EID of the result in the Scopus database
2-s2.0-85067808206