Potential of Smoke-Water and One of Its Active Compounds (karrikinolide, KAR(1)) on the Phytochemical and Antioxidant Activity of Eucomis autumnalis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00520741" target="_blank" >RIV/61389030:_____/19:00520741 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/19:73598950

Result on the web

<a href="http://dx.doi.org/10.3390/antiox8120611" target="_blank" >http://dx.doi.org/10.3390/antiox8120611</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/antiox8120611" target="_blank" >10.3390/antiox8120611</a>

Result language

angličtina

Original language name

Potential of Smoke-Water and One of Its Active Compounds (karrikinolide, KAR(1)) on the Phytochemical and Antioxidant Activity of Eucomis autumnalis

Original language description

Eucomis autumnalis (Mill.) Chitt. subspecies autumnalis is a popular African plant that is susceptible to population decline because the bulbs are widely utilized for diverse medicinal purposes. As a result, approaches to ensure the sustainability of the plants are essential. In the current study, the influence of smoke-water (SW) and karrikinolide (KAR(1) isolated from SW extract) on the phytochemicals and antioxidant activity of in vitro and greenhouse-acclimatized Eucomis autumnalis subspecies autumnalis were evaluated. Leaf explants were cultured on Murashige and Skoog (MS) media supplemented with SW (1:500, 1:1000 and 1:1500 v/v dilutions) or KAR(1) (10(-7), 10(-8) and 10(-9) M) and grown for ten weeks. In vitro regenerants were subsequently acclimatized in the greenhouse for four months. Bioactive phytochemicals in different treatments were analyzed using ultra-high performance liquid chromatography (UHPLC-MS/MS), while antioxidant potential was evaluated using two chemical tests namely: DPPH and the beta-carotene model. Smoke-water and KAR(1) generally influenced the quantity and types of phytochemicals in in vitro regenerants and acclimatized plants. In addition to eucomic acid, 15 phenolic acids and flavonoids were quantified, however, some were specific to either the in vitro regenerants or greenhouse-acclimatized plants. The majority of the phenolic acids and flavonoids were generally higher in in vitro regenerants than in acclimatized plants. Evidence from the chemical tests indicated an increase in antioxidant activity of SW and KAR(1)-treated regenerants and acclimatized plants. Overall, these findings unravel the value of SW and KAR(1) as potential elicitors for bioactive phytochemicals with therapeutic activity in plants facilitated via in vitro culture systems. In addition, it affords an efficient means to ensure the sustainability of the investigated plant. Nevertheless, further studies focusing on the use of other types of antioxidant test systems (including in vivo model) and the carry-over effect of the application of SW and KAR(1) for a longer duration will be pertinent. In addition, the safety of the resultant plant extracts and their pharmacological efficacy in clinical relevance systems is required.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ANTIOXIDANTS

ISSN

2076-3921

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

12

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

611

UT code for WoS article

000506633000044

EID of the result in the Scopus database

2-s2.0-85078625329