Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00522098" target="_blank" >RIV/61389030:_____/19:00522098 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-019-55652-3" target="_blank" >https://www.nature.com/articles/s41598-019-55652-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-019-55652-3" target="_blank" >10.1038/s41598-019-55652-3</a>
Alternative languages
Result language
angličtina
Original language name
Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars
Original language description
Sugarcane (Saccharum spp.) is a globally important crop for sugar and bioenergy production. Its highly polyploid, complex genome has hindered progress in understanding its molecular structure. Flow cytometric sorting and analysis has been used in other important crops with large genomes to dissect the genome into component chromosomes. Here we present for the first time a method to prepare suspensions of intact sugarcane chromosomes for flow cytometric analysis and sorting. Flow karyotypes were generated for two S. officinarum and three hybrid cultivars. Five main peaks were identified and each genotype had a distinct flow karyotype profile. The flow karyotypes of S. officinarum were sharper and with more discrete peaks than the hybrids, this difference is probably due to the double genome structure of the hybrids. Simple Sequence Repeat (SSR) markers were used to determine that at least one allelic copy of each of the 10 basic chromosomes could be found in each peak for every genotype, except R570, suggesting that the peaks may represent ancestral Saccharum sub genomes. The ability to flow sort Saccharum chromosomes will allow us to isolate and analyse chromosomes of interest and further examine the structure and evolution of the sugarcane genome.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
DEC 18
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
19362
UT code for WoS article
000507594500002
EID of the result in the Scopus database
2-s2.0-85076886234