Headspace gas chromatographic method for antimicrobial screening: Minimum inhibitory concentration determination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00524242" target="_blank" >RIV/61389030:_____/20:00524242 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/20:73604696

Result on the web

<a href="http://doi.org/10.1016/j.jpba.2020.113122" target="_blank" >http://doi.org/10.1016/j.jpba.2020.113122</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2020.113122" target="_blank" >10.1016/j.jpba.2020.113122</a>

Result language

angličtina

Original language name

Headspace gas chromatographic method for antimicrobial screening: Minimum inhibitory concentration determination

Original language description

The headspace gas chromatographic method has been widely used to detect volatile metabolites to reflect the growth state of microorganisms, however, it has never been used for the determination of the minimum inhibitory concentration in antibacterial drugs. This paper reports a new method for evaluating the antimicrobial activity of drugs by measuring the amount of CO2 produced by bacterial metabolism after treatment with drugs. According to the amount of CO2 produced by bacterial metabolism, a proper amount of bacterial liquid is selected and added to a drug-containing culture medium as compared with bacteria without drugs in parallel. The amount of CO2 produced by bacteria is measured by using a headspace gas chromatograph coupled with a thermal conductivity detector to measure the bacteriostasis rate and the minimum bacteriostasis concentration of the tested drug, so as to evaluate its antibacterial activity. The accuracy of this method was verified by comparison with the standard method (the OD method), which indicated that the precision was less than 3 % (expressed by relative standard deviation), the inhibition rate (R2 = 0.968) was consistent with the reference method above. This method is simple in operation and can avoid the error caused by the properties of the sample such as volatility, solubility and color in the determination of the minimum inhibitory concentration. It is suitable for the determination of antibacterial activity of drugs, especially natural drugs.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/EF16_019%2F0000868" target="_blank" >EF16_019/0000868: Molecular, cellular and clinical approach to healthy ageing</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

—

Volume of the periodical

181

Issue of the periodical within the volume

MAR 20

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

113122

UT code for WoS article

000516107400002

EID of the result in the Scopus database

2-s2.0-85078163505