Preparation and Standardisation of Smoke-Water for Seed Germination and Plant Growth Stimulation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00524406" target="_blank" >RIV/61389030:_____/20:00524406 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73604591
Result on the web
<a href="http://doi.org/10.1007/s00344-019-09985-y" target="_blank" >http://doi.org/10.1007/s00344-019-09985-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00344-019-09985-y" target="_blank" >10.1007/s00344-019-09985-y</a>
Alternative languages
Result language
angličtina
Original language name
Preparation and Standardisation of Smoke-Water for Seed Germination and Plant Growth Stimulation
Original language description
Smoke-water (SW) has a positive effect on seed germination in many plant species and its application in various fields of plant science has become popular. The method of preparing SW is relatively easy and inexpensive. However, many researchers working in the field of smoke biology are still unfamiliar with preparing SW and testing it for bioactivity. Thus, the aim of this study was to develop a low-cost efficient apparatus to produce SW using grasses, its standardisation and quantification of major active biomolecules. The prepared crude SW was diluted with distilled water (SW:DW) to 75:25, 50:50 and 25:75 (v/v) and these sub-dilutions were further diluted to 1:500 - 1:1000 - 1:1500 - 1:2000 - 1:2500 - 1:3000 and 1:3500 (v/v). For the standardisation of SW, lettuce seeds (Lactuca sativa L. cv. Grand Rapids) were tested to determine the best working concentration. The ratio 1:2500 (v/v) from the sub-dilution 25:75 (v/v) of SW was the best for germination of lettuce seeds in the dark, achieving 91% germination against the water control that had only 7–10% germination. This is the first study which reports the levels of stimulatory (karrikinolide 1, KAR1 and karrikinolide 2, KAR2) and inhibitory (trimethylbutenolide, TMB) compounds present in SW using ultrahigh-performance liquid chromatography–electrospray positive ionisation tandem mass spectrometry (UHPLC-ESI(+)-MS/MS).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Plant Growth Regulation
ISSN
0721-7595
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
338-345
UT code for WoS article
000521992300027
EID of the result in the Scopus database
2-s2.0-85067795221