Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531903" target="_blank" >RIV/61389030:_____/20:00531903 - isvavai.cz</a>

Result on the web

<a href="http://doi.org/10.1007/s00299-020-02544-w" target="_blank" >http://doi.org/10.1007/s00299-020-02544-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00299-020-02544-w" target="_blank" >10.1007/s00299-020-02544-w</a>

Result language

angličtina

Original language name

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Original language description

Key message: This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. nAbstract: Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell Reports

ISSN

0721-7714

e-ISSN

—

Volume of the periodical

39

Issue of the periodical within the volume

9

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

1115-1127

UT code for WoS article

000528435000001

EID of the result in the Scopus database

2-s2.0-85084142930