Homozygous transgenic barley (Hordeum vulgare L.) plants by anther culture
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00532719" target="_blank" >RIV/61389030:_____/20:00532719 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73604727
Result on the web
<a href="http://doi.org/10.3390/plants9070918" target="_blank" >http://doi.org/10.3390/plants9070918</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants9070918" target="_blank" >10.3390/plants9070918</a>
Alternative languages
Result language
angličtina
Original language name
Homozygous transgenic barley (Hordeum vulgare L.) plants by anther culture
Original language description
Production of homozygous lines derived from transgenic plants is one of the important steps for phenotyping and genotyping transgenic progeny. The selection of homozygous plants is a tedious process that can be significantly shortened by androgenesis, cultivation of anthers, or isolated microspores. Doubled haploid (DH) production achieves complete homozygosity in one generation. We obtained transgenic homozygous DH lines from six different transgenic events by using anther culture. Anthers were isolated from T0 transgenic primary regenerants and cultivated in vitro. The ploidy level was determined in green regenerants. At least half of the 2n green plants were transgenic, and their progeny were shown to carry the transgene. The process of dihaploidization did not affect the expression of the transgene. Embryo cultures were used to reduce the time to seed of the next generation. The application of these methods enables rapid evaluation of transgenic lines for gene function studies and trait evaluation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centre for Experimental Plant Biology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plants
ISSN
2223-7747
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
1-11
UT code for WoS article
000559483300001
EID of the result in the Scopus database
2-s2.0-85088267703