Isolation of High Purity Tissues from Developing Barley Seeds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00535186" target="_blank" >RIV/61389030:_____/20:00535186 - isvavai.cz</a>
Result on the web
<a href="http://doi.org/10.3791/61681" target="_blank" >http://doi.org/10.3791/61681</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/61681" target="_blank" >10.3791/61681</a>
Alternative languages
Result language
angličtina
Original language name
Isolation of High Purity Tissues from Developing Barley Seeds
Original language description
Understanding the mechanisms regulating the development of cereal seeds is essential for plant breeding and increasing yield. However, the analysis of cereal seeds is challenging owing to the minute size, the liquid character of some tissues, and the tight inter-tissue connections. Here, we demonstrate a detailed protocol for dissection of the embryo, endosperm, and seed maternal tissues at early, middle, and late stages of barley seed development. The protocol is based on a manual tissue dissection using fine-pointed tools and a binocular microscope, followed by ploidy analysis-based purity control. Seed maternal tissues and embryos are diploid, while the endosperm is triploid tissue. This allows the monitoring of sample purity using flow cytometry. Additional measurements revealed the high quality of RNA isolated from such samples and their usability for high-sensitivity analysis. In conclusion, this protocol describes how to practically dissect pure tissues from developing grains of cultivated barley and potentially also other cereals.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Jove-Journal of Visualized Experiments
ISSN
1940-087X
e-ISSN
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Volume of the periodical
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Issue of the periodical within the volume
164
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
e61681
UT code for WoS article
000596838300032
EID of the result in the Scopus database
2-s2.0-85095972756