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Isolation of High Purity Tissues from Developing Barley Seeds

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00535186" target="_blank" >RIV/61389030:_____/20:00535186 - isvavai.cz</a>

  • Result on the web

    <a href="http://doi.org/10.3791/61681" target="_blank" >http://doi.org/10.3791/61681</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3791/61681" target="_blank" >10.3791/61681</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Isolation of High Purity Tissues from Developing Barley Seeds

  • Original language description

    Understanding the mechanisms regulating the development of cereal seeds is essential for plant breeding and increasing yield. However, the analysis of cereal seeds is challenging owing to the minute size, the liquid character of some tissues, and the tight inter-tissue connections. Here, we demonstrate a detailed protocol for dissection of the embryo, endosperm, and seed maternal tissues at early, middle, and late stages of barley seed development. The protocol is based on a manual tissue dissection using fine-pointed tools and a binocular microscope, followed by ploidy analysis-based purity control. Seed maternal tissues and embryos are diploid, while the endosperm is triploid tissue. This allows the monitoring of sample purity using flow cytometry. Additional measurements revealed the high quality of RNA isolated from such samples and their usability for high-sensitivity analysis. In conclusion, this protocol describes how to practically dissect pure tissues from developing grains of cultivated barley and potentially also other cereals.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Jove-Journal of Visualized Experiments

  • ISSN

    1940-087X

  • e-ISSN

  • Volume of the periodical

  • Issue of the periodical within the volume

    164

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    e61681

  • UT code for WoS article

    000596838300032

  • EID of the result in the Scopus database

    2-s2.0-85095972756