Priming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00546175" target="_blank" >RIV/61389030:_____/21:00546175 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/21:73610711
Result on the web
<a href="http://doi.org/10.3390/plants10030446" target="_blank" >http://doi.org/10.3390/plants10030446</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants10030446" target="_blank" >10.3390/plants10030446</a>
Alternative languages
Result language
angličtina
Original language name
Priming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress
Original language description
In the context of global climate change, forest tree research should be addressed to provide genotypes with increased resilience to high temperature events. These improved plants can be obtained by heat priming during somatic embryogenesis (SE), which would produce an epigenetic-mediated transgenerational memory. Thereby, we applied 37 °C or 50 °C to maritime pine (Pinus pinaster) megagametophytes and the obtained embryogenic masses went through the subsequent SE phases to produce plants that were further subjected to heat stress conditions. A putative transcription factor WRKY11 was upregulated in priming-derived embryonal masses, and also in the regenerated P37 and P50 plants, suggesting its role in establishing an epigenetic memory in this plant species. In vitro-grown P50 plants also showed higher cytokinin content and SOD upregula-tion, which points to a better responsiveness to heat stress. Heat exposure of two-year-old maritime pine plants induced upregulation of HSP70 in those derived from primed embryogenic masses, that also showed better osmotic adjustment and higher increases in chlorophyll, soluble sugars and starch contents. Moreover, ɸPSII of P50 plants was less affected by heat exposure. Thus, our results suggest that priming at 50 °C at the SE induction phase is a promising strategy to improve heat resilience in maritime pine.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plants
ISSN
2223-7747
e-ISSN
2223-7747
Volume of the periodical
10
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
24
Pages from-to
446
UT code for WoS article
000634069800001
EID of the result in the Scopus database
2-s2.0-85101632269