Overexpression of rice OsWNK9 promotes arsenite tolerance in transgenic Arabidopsis plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00547646" target="_blank" >RIV/61389030:_____/21:00547646 - isvavai.cz</a>

Result on the web

<a href="http://doi.org/10.1016/j.jbiotec.2021.04.001" target="_blank" >http://doi.org/10.1016/j.jbiotec.2021.04.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jbiotec.2021.04.001" target="_blank" >10.1016/j.jbiotec.2021.04.001</a>

Result language

angličtina

Original language name

Overexpression of rice OsWNK9 promotes arsenite tolerance in transgenic Arabidopsis plants

Original language description

Protein kinases are involved in the transfer of phosphate group to serine, threonine, and tyrosine residues of a target protein. With No Lysine (WNK) kinase is a member of the serine/threonine protein kinase family, which has conserved catalytic lysine (K) residue in subdomain I instead of being in subdomain II.The WNKs family members in plants are stress inducible and have been validated for their role in abiotic stress tolerance. In the present study, we have characterized Arabidopsis overexpressed lines of OsWNK9 regulated by the constitutive promoter under arsenite stress. Moreover, we have performed In silico expression analysis of OsWNK9 under nutrient deficiency and heavy metal stress. Three independent transgenic Arabidopsis (OsWNK9-OX T11, T12,andT13) lines showed tolerance to arsenite stress compared to wild-type (WT) plants. Under arsenite stress, transgenic lines T11, T12 and T13 showed 56.46, 57.8 and 51.66 % increased biomass respectively, as compared to WT plants. All three ArabidopsisOsWNK9-OX lines exhibited higher proline content, increased antioxidant enzyme activities and lower hydrogen peroxide levels under arsenite stress. Besides, the total antioxidant capacity in terms of DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging percentage was increased by 8–15 % in three independent OsWNK9-OX lines compared with those of WT plants. Protein-protein interaction analysis of OsWNK9 predicted interaction partners with protein kinase and oxidative stress-responsive protein. Co-expression analysis of OsWNK9 in phosphate deficiency and arsenate stress condition predicted various proteins including membrane transporter and transcription factors. Taken together, our results, for the first time, provide evidence that OsWNK9 could positively mediate arsenite stress tolerance in plants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biotechnology

ISSN

0168-1656

e-ISSN

1873-4863

Volume of the periodical

332

Issue of the periodical within the volume

MAY 20

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

114-125

UT code for WoS article

000648620400002

EID of the result in the Scopus database

2-s2.0-85104647368