Cytokinins are involved in drought tolerance of Pinus radiata plants originating from embryonal masses induced at high temperatures

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00548997" target="_blank" >RIV/61389030:_____/21:00548997 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/21:73610167

Result on the web

<a href="http://doi.org/10.1093/treephys/tpaa055" target="_blank" >http://doi.org/10.1093/treephys/tpaa055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/treephys/tpaa055" target="_blank" >10.1093/treephys/tpaa055</a>

Result language

angličtina

Original language name

Cytokinins are involved in drought tolerance of Pinus radiata plants originating from embryonal masses induced at high temperatures

Original language description

Vegetative propagation through somatic embryogenesis is an effective method to produce elite varieties and can be applied as a tool to study the response of plants to different stresses. Several studies show that environmental changes during embryogenesis could determine future plant development. Moreover, we previously reported that physical and chemical conditions during somatic embryogenesis can determine the protein, hormone and metabolite profiles, as well as the micromorphological and ultrastructural organization of embryonal masses and somatic embryos. In this sense, phytohormones are key players throughout the somatic embryogenesis process as well as during numerous stress-adaptation responses. In this work, we first applied different higherature regimes (30 °C, 4 weeks, 40 °C, 4 days, 50 °C, 5 min) during induction of Pinus radiata D. Don somatic embryogenesis, together with control temperature (23 °C). Then, the somatic plants regenerated from initiated embryogenic cell lines and cultivated in greenhouse conditions were subjected to drought stress and control treatments to evaluate survival, growth and several physiological traits (relative water content, water potential, photosynthesis, stomatal conductance and transpiration). Based on those preliminary results, even more extreme higherature regimes were applied during induction (40 °C, 4 h, 50 °C, 30 min, 60 °C, 5 min) and the corresponding cytokinin profiles of initiated embryonal masses from different lines were analysed. The results showed that the temperature regime during induction had delayed negative effects on drought resilience of somatic plants as indicated by survival, photosynthetic activity and water- use efficiency. However, high temperatures for extended periods of time enhanced subsequent plant growth in well-watered conditions. Higherature regime treatments induced significant differences in the profile of total cytokinin bases, N6-isopentenyladenine, cis-zeatin riboside and trans-zeatin riboside. We concluded that phytohormones could be potential regulators of stress-response processes during initial steps of somatic embryogenesis and that they may have delayed implications in further developmental processes, determining the performance of the generated plants.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Tree Physiology

ISSN

0829-318X

e-ISSN

1758-4469

Volume of the periodical

41

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

912-926

UT code for WoS article

000671960000003

EID of the result in the Scopus database

2-s2.0-85108123677