Influence of different cytokinins on the phenolic acids and antioxidant activity of two Brachystelma species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00549509" target="_blank" >RIV/61389030:_____/21:00549509 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/21:73610744
Result on the web
<a href="http://doi.org/10.1007/s11240-021-02038-x" target="_blank" >http://doi.org/10.1007/s11240-021-02038-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-021-02038-x" target="_blank" >10.1007/s11240-021-02038-x</a>
Alternative languages
Result language
angličtina
Original language name
Influence of different cytokinins on the phenolic acids and antioxidant activity of two Brachystelma species
Original language description
The genus Brachystelma morphologically ranges from slender climbers to geophytes that mainly occur in Australia, Southeast Asia and sub-Saharan Africa. These species are well-known for their nutrition and medicinal values. The current study evaluated the effect of cytokinins (CKs) on the phenolic acid content and antioxidant activity of two in vitro-regenerated Brachystelma species. We quantified the phenolic acids (using Ultra-high performance liquid chromatography-tandem mass spectrometry, UHPLC-MS/MS) and antioxidant (Oxygen radical absorbance capacity, ORAC) potential of two (B. pulchellum and B. pygmaeum) species that were successfully micropropagated, with the use of three cytokinins (CKs): benzyladenine (BA), isopentenyladenine (iP) and meta-topolin riboside (mTR). The presence of CKs strongly influenced the concentrations of the 11 phenolic acids (consisting of seven hydroxybenzoic acids and four hydroxycinnamic acids) and ORAC of the in vitro-regenerated B. pulchellum and B. pygmaeum. Generally, the levels of hydroxycinnamic acids were higher than the hydroxybenzoic acids in both Brachystelma species. Ferulic (9-55 mu g/g DW) and sinapic (14-97 mu g/g DW) acids were the major phenolic acids in the micro-propagated plants. In both Brachystelma species, the majority of the CK-regenerated plants had higher ORAC relative to the control. The best antioxidant activity (> 70 mu mol/g Trolox equivalents, TE) was obtained from 25 mu M iP and mTR treatments for B. pulchellum and B. pygmaeum, respectively. Overall, the current study demonstrated the ability of CKs to serve as a suitable tool for the production of valuable therapeutic chemicals in the two Brachystelma species.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/EF17_048%2F0007323" target="_blank" >EF17_048/0007323: Development of pre-applied research in nanotechnogy and biotechnology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell, Tissue and Organ Culture
ISSN
0167-6857
e-ISSN
1573-5044
Volume of the periodical
145
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
689-699
UT code for WoS article
000622678500001
EID of the result in the Scopus database
2-s2.0-85101817171