Auxin requirements for a meristematic state in roots depend on a dual brassinosteroid function

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00552822" target="_blank" >RIV/61389030:_____/21:00552822 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/21:73610731

Result on the web

<a href="http://doi.org/10.1016/j.cub.2021.07.075" target="_blank" >http://doi.org/10.1016/j.cub.2021.07.075</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cub.2021.07.075" target="_blank" >10.1016/j.cub.2021.07.075</a>

Result language

angličtina

Original language name

Auxin requirements for a meristematic state in roots depend on a dual brassinosteroid function

Original language description

Root meristem organization is maintained by an interplay between hormone signaling pathways that both interpret and determine their accumulation and distribution. The interacting hormones Brassinosteroids (BR) and auxin control the number of meristematic cells in the Arabidopsis root. BR was reported both to promote auxin signaling input and to repress auxin signaling output. Whether these contradicting molecular outcomes co-occur and what their significance in meristem function is remain unclear. Here, we established a dual effect of BR on auxin, with BR simultaneously promoting auxin biosynthesis and repressing auxin transcriptional output, which is essential for meristem maintenance. Blocking BR-induced auxin synthesis resulted in rapid BR-mediated meristem loss. Conversely, plants with reduced BR levels were resistant to a critical loss of auxin biosynthesis, maintaining their meristem morphology. In agreement, injured root meristems, which rely solely on local auxin synthesis, regenerated when both auxin and BR synthesis were inhibited. Use of BIN2 as a tool to selectively inhibit BR signaling yielded meristems with distinct phenotypes depending on the perturbed tissue: meristem reminiscent either of BR-deficient mutants or of high BR exposure. This enabled mapping of the BR-auxin interaction that maintains the meristem to the outer epidermis and lateral root cap tissues and demonstrated the essentiality of BR signaling in these tissues for meristem response to BR. BR activity in internal tissues however, proved necessary to control BR levels. Together, we demonstrate a basis for inter-tissue coordination and how a critical ratio between these hormones determines the meristematic state.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10609 - Biochemical research methods

Project

<a href="/en/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centre for Experimental Plant Biology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Current Biology

ISSN

0960-9822

e-ISSN

1879-0445

Volume of the periodical

31

Issue of the periodical within the volume

20

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

4462-4472

UT code for WoS article

000711688200004

EID of the result in the Scopus database

2-s2.0-85118161304