Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CIOX proteins and cytokinin responses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00552834" target="_blank" >RIV/61389030:_____/21:00552834 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/21:73610743

Result on the web

<a href="http://doi.org/10.1016/j.molp.2021.07.015" target="_blank" >http://doi.org/10.1016/j.molp.2021.07.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molp.2021.07.015" target="_blank" >10.1016/j.molp.2021.07.015</a>

Result language

angličtina

Original language name

Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CIOX proteins and cytokinin responses

Original language description

Eukaryotic organisms are equipped with quality-control mechanisms that survey protein folding in the endoplasmic reticulum (ER) and remove non-native proteins by ER-associated degradation (ERAD). Recent research has shown that cytokinin-degrading CIOX proteins are subjected to ERAD during plant development. The mechanisms of plant ERAD, including the export of substrate proteins from the ER, are not fully understood, and the molecular components involved in the ERAD of CIOX are unknown. Here, we show that heavy metal-associated isoprenylated plant proteins (HIPPs) interact specifically with CIOX proteins synthesized in the ER and processed by ERAD. CIOX-HIPP protein complexes were detected at the ER as well as in the cytosol, suggesting that the complexes involve retrotranslocated CIOX protein species. Altered CIOX levels in HIPP-overexpressing and higher-order hipp mutant plants suggest that the studied HIPPs control the ERAD of CIOX. Deregulation of CIOX proteins caused corresponding changes in the cytokinin signaling activity and triggered typical morphological cytokinin responses. Notably, transcriptional repression of HIPP genes by cytokinin indicates a feedback regulatory mechanism of cytokinin homeostasis and signaling responses. Moreover, loss of function of HIPP genes constitutively activates the unfolded protein response and compromises the ER stress tolerance. Collectively, these results suggests that HIPPs represent novel functional components of plant ERAD.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Plant

ISSN

1674-2052

e-ISSN

1752-9867

Volume of the periodical

14

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

1918-1934

UT code for WoS article

000715075800016

EID of the result in the Scopus database

2-s2.0-85114248476