Impairment of root auxin-cytokinins homeostasis induces collapse of incompatible melon grafts during fruit ripening

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00562064" target="_blank" >RIV/61389030:_____/22:00562064 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/22:73616451

Result on the web

<a href="https://doi.org/10.1093/hr/uhac110" target="_blank" >https://doi.org/10.1093/hr/uhac110</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/hr/uhac110" target="_blank" >10.1093/hr/uhac110</a>

Result language

angličtina

Original language name

Impairment of root auxin-cytokinins homeostasis induces collapse of incompatible melon grafts during fruit ripening

Original language description

The factors underlying the plant collapse of certain melon-pumpkin graft combinations are not fully understood. Our working hypothesis was that impairment of photoassimilates transport in incompatible combinations induces an imbalance in the homeostasis of root auxin (indole-3-acetic acid, IAA) and of cytokinins, probably triggering plant collapse. Root IAA and cytokinins levels in the presence and absence of fruit and changes in root and scion metabolites were investigated in compatible and incompatible combinations. We showed elevated levels of IAA, 2-oxoindole-3-acetic acid (IAA catabolite), indole-3-acetylaspartate (IAA conjugate), and cis-zeatin-type cytokinins, but low levels of trans-zeatin-type cytokinins in the roots of plants of the incompatible combination during fruit ripening. Similarly, during fruit ripening, the expression of the YUCCA genes, YUC2, YUC6, and YUC11 (required for auxin biosynthesis), the GRETCHEN-HAGEN3 gene (required for auxin conjugation), and the cytokinin oxidase/dehydrogenase 7 (CKX7) gene (regulates the irreversible degradation of cytokinin) was enhanced in the roots of plants of the incompatible combination. Moreover, in the incompatible combination the fruiting process restricted transport of photoassimilates to the rootstock and induces their accumulation in the scion. In addition, high levels of hydrogen peroxide and malondialdehyde and reduced activity of antioxidant enzymes were observed in the roots of the incompatible graft. Our results showed that the collapse of the incompatible graft combination during fruit ripening is closely associated with a dramatic accumulation of IAA in the roots, which probably elicits oxidative damage and disturbs the balance of IAA and cytokinins that is of critical importance in melon-pumpkin graft compatibility.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Horticulture Research

ISSN

2662-6810

e-ISSN

2052-7276

Volume of the periodical

9

Issue of the periodical within the volume

JAN 5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

uhac110

UT code for WoS article

000828691700022

EID of the result in the Scopus database

2-s2.0-85135713255